Polyherbal formulation has been utilized all over the world because of many medicinal and therapeutic uses, without having any side effects. Individual plant active phytochemical components are insufficient to achieve the intended therapeutic effects, so making polyherbal formulation with a number of different plants shows better synergistic effect. The present study investigated the In-vitro and Ex-vivo permeability study individually of Dalbergia sissoo, Tagetes erecta, and polyherbal formulation (having combination of both the extracts) and compared with rutin suspension. The formulation was prepared with combination of hydroalcholic extract of leaves of Dalbergia sissoo and Tagetes erecta and evaluated.