This study was done in Sinnar- Central blood bank, Sinnar State- Sudan. Blood sample collected from apparent healthy blood donor during period of sample collection six months (October 2021-march 2022).This study aim to detect the incidence and factors associated with viral hepatitis (HBV&HCV)infections among blood donors in Sinnar State –Sudan. We conducted Hepatitis B surface antigen test strip and HCV Ab, as step to detect the infections among blood donors at the laboratories for central blood bank in Sinnar and confirmatory test of The HBsAg was analyzed by using sandwich ELISA in National Health Laboratory in Khartoum. The results of this study were analyzed statistically using the frequency and one sample T-test to find the significance of probability level according to SPSS ver20 program. (P) Value < 0.05 was considered significant. Among 7200 volunteer blood donors during sample collection (6 months), there were315 blood donors were positive by screening strip test, from all positive screening 107 included this study as cases and 78 negative blood donors were representing as control. Positive screening blood donors were included (100%) positive for HBsAg confirmatory test and highly significant HBsAg level (p˂0.000), 0(0%) for HCV Ab, the infection of HCV not detected among blood donors during this study. Percentage of infection was found to be high incidence in younger, high percentage at age group (18-28) years, also in West River Nile area, rural place, primary education and married blood donors.