ISSN : 2663-2187

Investigating the impact of salt and drought stress on germination and early seedling growth stages of Rapeseeds (Brassica napus L.)

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Hizia Kelaleche, Sihem Ziouche, Amel Bourahla, Chafia Benider
» doi: 10.33472/AFJBS.6.10.2024.5298-5310

Abstract

The most sensitive stage to abiotic stress is considered seed germination. These stressors comprise high soil salinity, drought, and irrigation water, which can negatively affect seeds’ germination and plant growth, especially in semi-arid and arid regions. Rapeseed is an oilseed crop adapted to the Mediterranean area; however, it is reportedly sensitive to salt and water stress during seed germination. In the present study, we tested under controlled conditions. Two randomized complete block factorial experiments were conducted under simulated drought and salinity stress conditions using polyethylene glycol PEG 6000 and NaCl, respectively. The experiment used different concentrations of saline solution, including 0 (distilled water), 50, 100, and 150 mmol.l-1 of NaCl. The following osmotic potentials were applied to simulate water deficit in PEG 6000: 0 (distilled water), 5%, 10%, 15%, and 20% (w/v). This study investigated Germination Percentage, Mean Daily Germination, Seedling Vigor Index, and root and shoot length. There were notable variations among the treatments (p<0.01) for all the traits. The impact of water and salt stress was responsible for a decrease in all the identified germination and seedling parameters. Increasing NaCl levels significantly impacted the germination process, particularly under salinity levels of 150, 200, and 250 mM NaCl and 20% PEG. A higher concentration of NaCl (250 mM) and PEG (20%) resulted in a significant decrease in rapeseed yield. Higher PEG and NaCl concentrations decrease shoot and root length. Osmotic stress affects seedling elongation more than germination percentage. This indicates that seedlings prioritize elongation over germination in response to osmotic stress.

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