Introduction-The most important climbing plant in the Menispermaceae family is Cissampelos Pareira. It was used as a traditional medical method in the past to cure a variety of ailments such ulcers, wounds, fevers, asthma attacks, cholera, diarrhea, inflammation, snakebites, malaria, and rabies. It was also advised for the purifying of blood. Aim and objective: This review's goal is to enlighten readers on the phytochemistry, chromatographic and spectroscopic analyses, pharmacology, toxicity, and other aspects of Cissampelos pareira, as well as potential areas for further investigation. This knowledge provides the way for the discovery of future plant-based drugs. Cissampelos Pareira literature is gathered via online databases as scifinder, Web of Science, PubMed, and Google Scholar. published books were searched for details about this plant. Our study focuses on Cissampelos Pareira and employs botany, chemistry, and phytochemistry. There were 54 plant compounds found, including fatty acids, iso-quinoline alkaloids, flavonoids, and flavonoid glycosides. Method- Cissampelos pareira crude extract exhibits various pharmacological activities, including antipyretic, antiulcer, antidiabetic, anti-inflammatory, anticancer, antifertility, antioxidant, and immune-dilator properties. The chemical fingerprinting of Cissampelos pareira, using various techniques like HPTLC, HPLC, UPLC, LC-MS, and GC-MS, revealed the presence of alkaloids, fatty acids, and flavonoid glycosides. Result- A review of the literature reveals this plant's considerable advancements in phytochemistry and pharmacology, emphasizing its therapeutic promise. Modern analysis has helped to clarify several traditional applications, but further research is needed to confirm the relationship between certain phytoconstituents and pharmacological actions. There is some information about toxicological testing and pharmacological investigations. Summary and conclusion- Most pharmacological studies on Cissampelos pareira have partial data and incomplete toxicological screening. Pre-clinical and clinical pharmacology research should be addressed in the future, with clinical trials conducted after determining a drug's safety, effectiveness, and mechanism of action.