An essential part of the traditional Indian Ayurvedic medical system was guggul. Belonging to the Burseraceae family, Commiphora wightii Bhandari (Syn. Commiphora mukul Hook. ex. Stocks). It has been used in Ayurveda since the beginning of time to treat a variety of diseases, including those with anti-inflammatory, antibacterial, anti-obesity, antioxidant, and antifungal properties. This plant is widely used in traditional Indian medicines. Based on historical usage and literary information, this plant was chosen for its potential to heal wounds. The study evaluated the ability of based on solvents bark extracts to heal wounds in vitro. The results of the phytochemical analysis showed that pure bark extracts contained every phytochemical component. It was discovered that the invitro wound healing effectiveness depended on both concentration and time. The highest level of effectiveness was observed at 100 μg/ml for a 36 h period, and the average area of the wound was recorded at 25 μg/mL during the same time frame. The current study found that, while the wound diminution area level was observed at lower concentration, the in-vitro wound healing activity result proved 100% wound healing cell migration effect at the maximum concentration at a maximum time period of 36 h. Significant wound healing activity, tissue regeneration, and inflammation regulation are found in the ethanol-based plant bark extracts. The idea that Commiphora wightii bark ethanolic extract exhibits crucial wound healing was also supported by histological analysis.