Arthritis encompasses various autoimmune conditions characterized by joint inflammation, includes rheumatoid arthritis. There is a growing interest in natural remedies alongwith non pharmacological interventions like hydrotherapy and acupuncture. Raphanuss ativus (radish, family: Brassicaceae) is recognized for its anti arthritic properties and potential health benefits. This study aims to explore the anti arthritic potential of Raphanussativus by in vitro and in vivo approach. Raphanussativus leaves were id entified, collected, and authenticated, followed by prepared fresh leaf juice. Phytochemical tests were conducted to identify secondary metabolites in the leaf juice. The i n vitro pharmacological approach of fresh leaf juice of Raphanussativus (RSLJ) was d one by Inhibition of protein denaturation method using BSA method and Inhibition of albumin denaturation by Egg Albumin Method. In vivo method involved the administration of RSLJ (200 and 400 mg/kg BW P.O ) to rats with formaldehyde (0.1 ml, subplantar inj e ction ) induced arthritis for 14days, assessed parameters like body weight changes, haematological and biochemical parameters, and radiological alterations. The phytochemical analysis revealed the presence of alkaloids, glycosides, tannins, and flavonoids i n RSLJ. The in vitro anti arthritic activity of RSLJ was significant, with dose dependent inhibition of protein denaturation comparable to diclofenac sodium. In FIA induced arthritic rats, treatment with RSLJ demonstrated significant alterations in body we ight, RBC WBC, Hb%, ESR, C reactive protein ( Rheumatoid factor (RF) and uric acid . Radiological assessment showed attenuation of joint abnormalities with low and high doses of Raphanussativus leaf juice. The study highlights the potent anti arthritic effects of RSLJ, attributing them to its phytochemical constituents such as alkaloids and flavonoids. Further research is warranted to elucidate its mechanisms and active constituents fully, and the leaf juice could be subjected to further isolation for identifying potent phytochemical constituents.