Carthamus tinctorius, commonly known as safflower, possesses diverse pharmacological properties. This study aimed to isolate and characterize lupeol, a bioactive compound, from both the leaves and flowers of Carthamus tinctorius. Results: Employing a combination of solvent extraction and chromatographic techniques, lupeol was successfully isolated. Structural elucidation was performed using Fourier Transform Infrared (FTIR) Spectroscopy, Proton nuclear magnetic resonance (1H-NMR),Carbon-13 (C13) nuclear magnetic resonance (13C-NMR) and mass spectrometry analyses. The finding confirms the isolation of lupeol from leaves and flowers of Carthamus tinctorius. Conclusions: This investigation shades light on the potential therapeutic applications of lupeol as a natural product derived from Carthamus tinctorius