The emerging resistance of pseudomonas aeruginosa to several antibiotic groups has resulted in difficulty in treating infected patients. As a result, bacteriophage therapy is now considered a promising treatment substitute. In this study, a novel bacteriophage, PaMAsh, with lytic activity against P. aeruginosa was isolated from wastewater. PaMAsh exhibited a moderate host range against the four tested bacterial strains, as well as high stability over a wide range of pH and temperature. Electron microscopy revealed that the PaMAsh phage belongs to the family Podoviridae.