Parkinson’s disease is a progressive neurological disorder that affects the nerve cells in the brain that produce dopamine. L-3, 4-dihydroxyphenylalanine (L-DOPA) is a natural precursor for the production of dopamine by the central nervous system required for normal movement of the body. Microbial tyrosinase by a series of reactions produces L-DOPA as an intermediate. The present work emphasizes on the isolation and characterization of potential bacteria with the ability of L-tyrosine to L-DOPA biotransformation from industrially contaminated soil. The contaminated soils from different industries of the Mandsaur and Neemuch district of Madhya Pradesh and the Chittorgarh district of Rajasthan were exploited for the present study. Out of the 48 isolates, seven were found with prominent tyrosinase production activity. Among these 7 strains, MUTY-5 is found to be the most potent tyrosinase-producing strain with tyrosinase production indexing 3.07±0.18. Microbial strain MUTY-5 is further characterized by biochemical and molecular methods. Based on 16S rRNA gene sequencing, we have identified tyrosinase-producing strain as Bacillus cereus. The study indicates the potential of Bacillus cereus MUTY-5 isolated from industrially contaminated soil for extracellular tyrosinase enzyme production which further can be explored for the transformation of L-tyrosine into L-DOPA under stress conditions.