The technology based on the use of micro-organisms, in this case bacteria isolated from sites contaminated by industries, represents an alternative option for the treatment of wastewater before discharge into the environment. The present study deals with isolation, identification and characterization of the unknown bacteria present in the industrial effluent. A total of nine bacteria were isolated from industrial effluent using nutrient agar medium. Based on the morphological, biochemical the isolated bacteria were identified as the species of Pseudomonas and E. coli. In this context we also tested the isolated bacteria for minimum inhibitory concentration and we found: Zenflox, Azithromycin and Erythromycin were showing maximum MIC value for Pseudomonas compared with E. coli. While in another set of experiment we found Ofloxacin has maximum MIC value for Pseudomonas, this result reveals that normal micro flora is also developing drug resistance activity