Volume 7 | Issue - 1 articles in press
Volume 7 | Issue - 1 articles in press
Volume 7 | Issue - 1 articles in press
Volume 7 | Issue - 1 articles in press
Volume 7 | Issue - 1 articles in press
The aim of the present investigation is to isolate the active phytoconstituents through bioactive guided approach from the chloroform extract. MATERIAL & METHODS- The fresh leaves of C. camphora were collected from outfield during the month of July that shows the green color with rough surface. Defatted drug was subjected to extraction with chloroform, ethyl acetate, ethanol and finally by using water. Preliminary phytochemical screening of different extracts for the presence of various active phytoconstituents was performed. Chloroform extract (50 g) was dissolved in a minimum volume of chloroform and adsorbed on silica gel (60-120 mesh), dried and applied on the column to separate possible phytoconstituents. Column was first eluted with pure n-hexane and then gradually with increasing quantity (95:5, 90:10, 85:15, 80:20, 75:25, 70:30, 65:35 and finally 50:50) of ethyl acetate. The fractions showing same TLC pattern were pooled together and finally 5 fractions (F1-F5) were obtained. On In vitro anti-diabetic evaluation of all collected fractions, Fraction-2 (F2) showed highest activity and single spot in TLC, it showed the strong presence of terpenes. RESULTS & DISCUSSION- One of the distinctive features in the IR spectrum of Linalool is the presence of a sharp and intense peak in the range of 3400 cm^-1, indicative of the O-H stretching vibrations associated with the hydroxyl group. This peak signifies the alcohol functionality inherent in Linalool. One prominent feature in the IR spectrum of borneol is the broad peak in the region around 3400 cm^-1, indicative of O-H stretching vibrations. The singlet at δ 1.39 corresponds to three equivalent protons in a methyl group, indicating the presence of aliphatic methyl moieties in linalool. CONCLUSION- From the chloroform extract, two active phytocompounds i.e. Borneol and Linalool were isolated and characterized.