This study aims to evaluate the level of knowledge, attitudes, and factors influencing skin and eye donation among patients in a tertiary care hospital. Methodology: A cross-sectional study was conducted at the tertiary teaching hospital’s outpatient dermatology and ophthalmology department. A total of 340 patients were included, and data were collected using structured questionnaires. Demographic characteristics, knowledge, attitudes, and factors influencing the eyes and skin donation were assessed. Results: Out of 340 participants the eye donation, 58.82% exhibited good knowledge, while 38.82% showed excellent knowledge. In contrast, for skin donation, 47.94% demonstrated average knowledge, and 18.82% exhibited good knowledge. Attitudes toward donation were generally positive, with a significant proportion agreeing that donation positively impacts someone's life (67.18%) and reflects positively on character (64.12%). No significant relationship was found between most demographic variables and knowledge scores for eye donation, except for family status. For skin donation, significant associations were found only with gender and religion. Conclusion: Targeted educational interventions are needed to enhance knowledge and attitudes towards eye and skin donation among patients in tertiary care hospitals. While positive attitudes towards donation were prevalent, tailored approaches are necessary to address knowledge gaps and demographic disparities.