This study investigates the anti-inflammatory effects of Lactobacillus gel in male Wistar rats with carrageenan-induced paw edema. The study compared the anti-inflammatory capabilities of Lactobacillus casei and Lactobacillus acidophilus in an acute inflammation model induced by carrageenan, using diclofenac sodium at 150 mg/kg as the control standard. The oral dosage of Lactobacillus bacteria administered was 2 × 10¹ CFU/ml. Rats were administered carrageenan to induce edema one hour after oral treatment, and paw thickness was measured at 1, 2, 3, 4, 5, and 24-hour intervals. Motility and stair climbing ability were assessed at 24-hour intervals, and serum samples were analyzed for IL-6, IL-10, and TNF-α cytokines. Lactobacillus treatment significantly reduced paw thickness (P < 0.001), with L. acidophilus and L. casei showing 34% and 30% reductions, respectively. Both strains improved motility and stair climbing ratings. Treatment with Lactobacillus notably decreased TNF-α and IL-6 levels while increasing IL-10 levels significantly (P < 0.0001). However, L. acidophilus and L. casei showed less pronounced effects on reducing carrageenan-induced inflammation, suggesting a potential dampening effect on pro-inflammatory cytokine production by Lactobacillus.