Tuberculosis is the 10th leading cause of deaths worldwide. In chronic infectious diseases inflammation is common rule and pulmonary tuberculosis (PTB) is one of them. Innate and adaptive immune system of host activates immune system specially T-cells which released cytokines. Cytokines altered iron homeostasis and reduced erythropoietin production which play a vital role in Patho-physiology of anaemia. Methods: The present study was an observational type of case control study. This study included 50 sputum Acid-Fast Bacilli (AFB) confirmed newly diagnosed pulmonary tuberculosis anemic PTB subjects as case and 50 sputum AFB confirmed newly diagnosed pulmonary tuberculosis non-anemic PTB subjects as controls. Serum EPO were analyzed by ELISA method and serum Iron were observed by ferrozine method in both groups. Results: Significantly lower levels of EPO (47.28 ± 6.40) were observed in anemic PTB cases than that of non-anemic PTB cases (p<0.001). Iron profile was also found significantly lower in anemic PTB group (24.85 ± 9.32) than non-anemic PTB group. Conclusion: The dynamic changes in iron status, Erythroferrone and EPO during resolution of acute inflammation of anemia caused by tuberculosis