Introduction: The principal androgen controlling spermatogenesis in the testis is T. By diffusing into the seminiferous tubules, which are home to the Sertoli cells, it functions as a paracrine factor. It is secreted by Leydig cells as a reaction to the luteinizing hormone (LH) signal. Aminopeptidase N (APN) is an enzyme of semen that is frequently present in multiple cell types and is linked to a number of illness states, including cancer, hypertension, neoplasm, inflammation, and obesity. Human seminal plasma has a 20-fold higher APN activity than brain cells.