Gardenia gummifera Linn. f. belongs to the family Rubiaceae and is one among the red-listed plants native to peninsular India. This study aims to evaluate its hepatoprotective effect against paracetamol-induced liver toxicity in a rat model. groups of rats were orally treated (once daily) with normal saline, paracetamol 640 mg/kg (negative control), 10 mg/kg silymarin (positive control), extract (100 and 200 mg/kg, and isolated compound (Gardenin A ) for 14 days, followed by the hepatotoxicity induction using paracetamol (PCM). The blood samples and livers of the animals were collected and subjected to biochemical and microscopical analysis. The histological findings suggest that paracetamol caused lymphocyte infiltration and marked necrosis, whereas maintenance of the normal hepatic structural was observed in group pre-treated with silymarin and Gardenin A. The rats pre-treated with methanol extract (200 mg/kg) and Gardenin A significantly and dose-dependently reduced the hepatotoxic markers in animals. methanol extract (200 mg/kg) and Gardenin A 10 mg/kg significantly reversed the paracetamol-induced altered situations, including the liver enzymes, antioxidant enzymes and serum bilirubin, levels in animals. Taken together, Gardenin A exerted significant hepatoprotective activity in rats in a dose-dependent manner. paracetamol induced toxicity and Gardenin A induced hepatoprotective effects based on expression of inflammatory and apoptosis factors will be future line of work for establishing the precise mechanism of action of Gardenin A in Wistar albino rats.