Anxiety is defined as a central nervous system disorder in which a patient suffers from an emotional state of mind, discomfort, uneasiness and fear about future. It is thus explained as mental disorder in which pathological anxiety causes disturbance of mood including thinking, behaviour & physiologicals. Anxiety is linked to a number of conditions, including obsessive-compulsive disorder, terror illness, anxiety, general panic disorder, a particular phobia, social phobia, acute psychological illness, and post-traumatic stress syndrome. among others. There are other problems resulting from substance-induced anxiety disorders,general medical illnesses, and adjustment disorders having anxiety symptoms. Nowadays, lot of interest has been generated in traditional and folklore medicines as researchers consider phyto-constituents as more effective in treating various diseases with minimal side effects. Thus there is a drift from synthetic ways to traditional and natural ways to fulfil the various health care needs. One such plant is A. pungens HB&K also called khaki weed that comes in botanical family Amaranthaceae which is studied under the present study. The traditional systems of medicines explore plants as the major weapon in healing different types of ailments).The phytoconstituents present in the plant are saponins, alkaloids, steroids, triterpenoids, flavonoids, anthraquinones, tannins, cardiac glycosides, coumarins, leucoanthocyanins. The biomarkers are isolated using different techniques from naturally occurring sources. With the advancement of modern techniques and organic chemistry man started synthesizing synthetic medicines using different types of substitution reactions by using naturally isolated compounds as a template.