Monitoring of antibiotic resistance patterns continuously is required because of the evolution of new bacterial species and empirical antimicrobial therapy. A prospective study was conducted in a tertiary care teaching hospital with an inclusion of 688 patients. Among688patients314patients were found with Surgical Site Infections (SSIs) with an incidence of 45.6. SSIs were more common in females(51.6%) than males(48.4%), the infection rate was found to be higher in emergency procedures when compared to planned elective procedures (P<0.05, by using Chi-square test; OR=0.08). The surgical procedures of <45min has led to the development of more SSIs. Out of 314 SSIs, 328 organisms were isolated, of which Staphylococcus–97 was highest and majority of it were highly resistant to Ceftriaxone (84.5%) and highly sensitive to Gentamicin (92.8%). Streptococcus isolates were highly resistant to Ciprofloxacin (63.04%) and highly sensitive to Gentamicin (91.3%). Gentamicin showed good activity against most of the organisms, Ceftriaxone was commonly used before and after surgery but it was ineffective against most organisms. There is a need to emphasize the use of antimicrobials rationally to minimize the misuse of available antimicrobials. In addition to that there is an essentiality for regular antimicrobial susceptibility surveillance among area-wise monitoring of resistance patterns.