Stenotrophomonas maltophilia is a nonfermenting Gram-negative rod that is ubiquitous in nature. This pathogen is frequently responsible for nosocomial outbreaks, especially in intensive care units (ICU). Stenotrophomonas maltophilia infections are associated with a high mortality in case of pneumonia and septicemia. Objective: Study focused on detects prevalence and antibiotics sensitivity of Stenotrophomonas maltophilia. Method: A study conducted at Microbiology Department of Smt. B. K. Shah Medical Institute and Research Centre, Piparia from January 2023 to December 2023. Culture for all the samples was done on sheep blood agar and MacConkey agar followed by Incubation at 37°C for 18–24 hours in 5–10% CO2. Bacterial isolates identified by standard laboratory procedure and antibiotic sensitivity was done by VITEK 2 compact system of bioMerieux. Results: 23 isolates of S. maltophilia were identified from various samples, with maximum number from sputum (n = 11, 47%) followed by blood (n = 6, 26.08%). Most of the patients (n = 13, 56.52%) were in the age group of 41–60 years. Predominant presenting symptoms were observed as respiratory tract infections 14 (60.87%). The isolates had high susceptibility to trimethoprimsulfamethoxazole (TMP-SXT) (91.3%) and Minocycline (60.9%). Conclusion: It is very important to give pathogen specific antibiotics as per antibiotics sensitivity testing, because it has shown to decrease the patient stay in the hospital and also decrease the mortality rate. New treatment guidelines recommend combination of antibiotics to obtain synergic effect.