The current study aims to study and diagnose Trichomoniosis among women in the northern regions of Basra Governorate (Medina District, Qurna District, Imam al-Sadiq District, Bani Mansour, Al-Huwair, Al-Khass, Al-Sharsh, Al-Deir) using the polymerase chain reaction (PCR) method and comparing it to the microscopy method. Classic and explain the relationship between infection and diseases of the female reproductive system and the demographic nature of patients. In this study, 100 samples were collected during the period that extended from August 2022 to February 2023, each of vaginal secretions, urine, and blood serum, from exclusively married women, whose ages ranged between 16-50 years, visiting outpatient clinics in the northern areas of Basra Governorate, and who suffer from gynecological and sewage infections. Urinary tract or more severe symptoms such as uterine enlargement or cervical ulcers.