Diversity of species arises majorly from speciation and this has serious epidemiological implications to disease transmission. The study aimed to determine the species of anopheline vectors of malaria present in five locations along with different altitudes on the highlands of Mambilla Plateau Nigeria. These are; Nguroje (1,885 m), Yelwa (1,674 m), Gembu (1,584 m), Kakara (1,496 m) and Mayo-selbe (484 m) above sea level.