The aim of the study was to identify the diversity of gut microbiota and the role diet in shaping the gut microbiota of healthy individuals from rural region of Haryana, North India. Methods: The study was conducted among healthy individuals within the age group 18-55 years from two villages (Kaliawas and Sultanpur) of Haryana. Data pertaining to their diet habits were recorded in predesigned questionnaire, after obtaining informed consent. Subjects were asked to provide stool sample and the samples were processed by both culture dependent and culture independent method to identify both aerobic and anaerobic bacterial isolates. Results: Stool samples from both the villages were dominated with phylum Firmicutes (Lactobacillus), followed by phylum Actinobacteria (Bifidobacterium), Bacteroidates (Prevotella and Bacteroides), Rhuminicoccus and Proteobacteria (E.coli). Altered flora (Salmonella and Yersinia) was seen among preserved food consumers. Higher abundance of Prevotella and significant Bacteroides were found among vegetarians and non vegetarians respectively. Significant difference of Lactobacillus was seen among those individuals between both the villages. Conclusion: To conclude, majority of the individuals from Haryana are dominated by beneficial bacterial flora due high intake of diary products, fermented food along with mixed diet. Present study suggests that individuals from rural region of Haryana might be counted as donar for Fecal microbiota transplant. It also emphasis on that more and more research needs to be conducted to understand the diversity and basic functional attributes of the gut microbiome of Indian population particularly from different regions of Haryana.