Multiplex polymerase chain reaction (PCR) is an important tool of modern molecular biology because it offers several important advantages over amplifications involving one DNA marker at a time (singleplexes). The use of multiplex PCR requires careful design of the primers for the loci of interest and rigorous optimization of cycling conditions to obtain results. The aim of this study was to design, construct and optimize multiplex PCR assays for the 10 STR loci using simple tools. DNA was extracted from 5 human blood samples obtained from Nigerian individuals. Primer sequences were obtained from literature. Ten (10) loci were combined into multiplex assays and tested by PCR followed by polyacrylamide gel electrophoresis and silver staining. The ten loci were successfully combined into four multiplexes M01 (Amelogenin and D10S1248), M02 (D6S1017 and D18S51), M03 (D22S1045, D21S11, and D2S1338), and M04 (D9S2157, FGA and D8S1179). A 20bp DNA ladder was used as DNA size marker while the gels were scored using the Gel Analyzer software. Generally, loci amplification improved with DNA concentration above 2ng, MgCl2 concentration between 1.5- 4.5mM, and 30-35 PCR cycles with final extension times between 10-30 minutes.