Urinary tract infections are a serious health issue that affects individuals of all ages. After respiratory tract infections, urinary tract infections rank as the second most common illness. People with diabetes experience more UTI-related issues than people without diabetes because bacteria can easily grow in the glucose-supplied medium. Aims and Objectives: The purpose of this study is to identify and evaluate the presence of uropathogens in urinary tract infected patients amongst diabetics and non-diabetics. Material and Methods: In this comparative study clinical and microbiological aspects of UTI were compared with diabetic and non-diabetic patients.UTI screening was conducted with midstream urinary samples and demographic characteristic, clinical profile and microscopy findings were recorded. Data were input and analyzed by using STATA 14.2 for comprehensive statistical evaluation. Results: 347 individuals reported positive for UTI out of 1048 total samples. 106 (30.5%) of them were non-diabetics and 241 (69.5%) were diabetics. The risk of UTI was higher in diabetic than non-diabetic group. The most prevalent uropathogens in both non-diabetics (48%) and diabetics (40%) was E. coli. Pseudomonas spp. 15% in diabetics and 10% in non-diabetics and Candida spp. 15% in diabetics and 9% in non-diabetics. Conclusion: Frequency of Urinary tract infections are more in diabetics than non-diabetics. Occurrence of E.coli was found more in non-diabetic compare to diabetic (48%vs40%). Where as Candida spp. was more prevalent in diabetic compare to non-diabetic (15%vs09%). Isolation rate of Pseudomonas spp. was higher in non diabetic compare to diabetic(10%vs03%). Higher prevalence of UTI was present in non-diabetic female compare to diabetic female (60.3%vs55.60%) and it was higher in diabetic male compare to non-diabetic male (44.3%vs39.6%).