The indiscriminate use of various pesticides causes contamination of different biological systems and accumulates in food chains and crops, thereby resulting in several health hazards for humans. Monocrotophos is a hazardous pesticide used globally, and thus its biodegradation is needed at this time. In this study, HR-115 (bacterial strain) was evaluated for its monocrotophos degradation potential using high-pressure liquid chromatography (HPLC). Five parameters inoculum size, pH, rpm, time, and temperature were optimized using response surface methodology to enhance the strain's ability to degrade the pesticide in the minimum incubation period. The model was found to be fit with an R² of 0.97, showing nonsignificant lack of fit and P-values less than 0.0500. The potent degrader HR-115 was further employed for Sanger sequencing and identified as Pseudomonas stutzeri (MRCJ-1020). HPLC results revealed a pesticide degradation potential of 64.89% after 72 hours of incubation.