The work optimised selective media to fine-tune culture media for stressed bacteria resuscitation in a cost-effective and potent way. Resuscitating exhausted microorganisms is crucial in microbiology. Stress alters metabolism, membranes, and cell walls, limiting culturability. In microbiology, biotechnology, and medical research, Escherichia coli (E. coli), a model organism, must recover successfully. Revitalization requires optimising nutrients, pH, osmotic balance, and antibiotics in the recovery medium. This optimisation mimics natural circumstances, encourages target microorganism growth, and improves research efficiency, cost, and sustainability. We found that Pancreatic Digest of Casein (A), Papaic Digest of Soybean (B), Sodium Chloride (C), and Dextrose Monohydrate (D) help stressed bacteria recover. Our Box-Behnken Design with twenty-nine experiments produced polynomial equations, ANOVA, and 3D response surface graphs. ANOVA confirms that these models accurately predict CFUs (Y1) and % recovery (Y2).We optimised these aspects using desirability. The ideal factor levels are: A: 3.90 mg/ml Pancreatic Digest of Casein, B: 1.90 mg/ml Papaic Digest of Soybean, C: 2.50 mg/ml Sodium Chloride, D: 0.90 mg/ml Dextrose Monohydrate. Experimental confirmations confirmed these parameters. The procedure's accuracy in determining optimal media composition is shown by the close match between anticipated and observed values. Optimisation of recovery medium composition is crucial for reviving stressed microorganisms. Improves research efficiency, cost-effectiveness, and environmental responsibility while increasing target microorganism output. It is vital to microbiological research and supports many scientific fields.