Pteridophytes commonly known as ferns play a significant part in traditional medicine, while being ignored nowadays. The primary goal of this study project was to screen various unexploited pteridophyte plants Pteris pellucens and Pteris grandifolia for their in vitro antioxidant and in vivo analgesic activity. The pharmacognostic standardization of the selected species of pteris was done as per standard protocols. The whole plant powders were extracted with ethanol, flavonoid rich fractions of Pteris pellucens (PPFRF) and Pteris grandifolia (PGFRF) were separated and arsenic content was estimated from their ethanolic extracts as pteris species were known to be hyper accumulators of arsenic. In-vitro antioxidant activity of ethanol extract and flavonoid rich fraction of the plant species under study were investigated using Superoxide radical scavenging, DPPH radical scavenging, Hydroxyl radical scavenging and Ferric reducing antioxidant power activity. The in vivo antinociceptive efficacy of the two plant extracts were analyzed using hot plate, tail flick and acetic acid-induced writhing test. In the powder microscopic studies of the studied plant extracts, V-shaped vascular bundles, a unique characteristic of pteridophytes, was found. Ethanolic extract of both the plantswere found to contain alkaloids, steroids, carbohydrates, glycosides, phenolic compounds, flavonoids and triterpenoids. Flavonoid rich fraction of the the species exhibited better antioxidant activity when compared to their corresponding ethanolic extracts. Comparing the plant extracts' flavonoid rich fractions to their ethanol extracts, it was noteworthy that the former showed significant antioxidant effects. The findings showed that the plant extracts exhibited significant reductions in thermal stimuli and writhing in a dose-dependent manner, indicating their effectiveness in reducing pain perception. These extracts were shown to possess antioxidant phytocompounds with a variety of pharmacologic actions, particularly via reducing oxidative stress, which accounted for their antinociceptive and in vitro antioxidant efficacies. To accelerate the design of accessible, affordable, secure, and effective medications, more research on the antinociceptive and antioxidant mechanism(s) as well as the isolation and characterization of relevant compounds is encouraged. Based on the results, we can conclude that the current study implies that this fraction could be very important in the treatment of free radical-related health issues and illnesses associated with age.