Water is a crucial necessity in public health, and the presence of safe water directly impacts the overall quality of life, as it is an essential element for survival. The objective of the present study was to investigate the physicochemical properties and antibiotic resistance patterns of bacterial species obtained from drinking water sources in certain communities within the Ishielu local government area of Ebonyi State. Water sources, specifically streams, rivers, and wells, were gathered for the purpose of conducting Physicochemical and bacteriological analysis using established analytical methods. The bacterial isolates were identified and characterised using conventional microbiological techniques. The physicochemical studies showed that the alkalinity, BOD, COD, pH, TH, TOC, DO, and turbidity values in the water samples (measured in mg/L) were all within the recommended limits for drinking water quality set by the World Health Organisation (WHO) and Nigeria standard for drinking water quality (NSDWQ), except for the alkalinity value in Azuinyaba ward. The bacteriological count results showed that water samples from diverse sources had a Total Bacterial count (TBC) of 9.10 x 10-4, 8.10 x 10-4, and 4.10 x 10-4, respectively. There was a statistically significant difference (p <0.05) in the bacterial load levels of water samples from all research locations during the study period. Prevalence investigations indicated that Escherichia coli had the highest occurrence, followed by Klebsiella species, and the least common was Salmonella species. The antibiotic investigations demonstrated that Salmonella, Klebsiella species, and E. coli exhibited significant resistance to some commonly used antibiotics, however they also displayed some vulnerability to certain drugs. Nevertheless, the significant presence of enteric bacteria and their high resistance value observed in this study strongly suggests that the samples were contaminated with faecal matter due to inadequate hygiene and sanitation practices in the respective locations. Furthermore, this finding indicates that the isolated bacteria have been exposed to multiple antibiotics, leading to the development of resistance within their bodies when infected with waterborne diseases.