Cyclodextrins (CDs) are natural compounds with a cyclic structure composed of 6-to-8 glucopyranose units. They have a rich history dating back to their recognition for inclusion complex formation in 1938 and pharmaceutical patent filings in 1953. CDs have a well-established safety profile, allowing their use in drug products. Different CD types, including α-CD, β-CD, and γ-CD, have varying properties and applications. β-CD is widely used in pharmaceuticals for enhancing drug solubility and stability, while γ-CD is ideal for poorly water-soluble drugs. CDs play a crucial role in scaffold development, tissue regeneration, and additive manufacturing. They can reinforce hydrogels, self-assemble into supramolecular networks, and serve as depots for disease management and tissue repair. CD-modified scaffolds facilitate prolonged gene vector delivery and contribute to additive manufacturing by enhancing bioinks and enabling rapid photopolymerization. CDs have a cone shape with a hydrophilic outer surface and hydrophobic cavity, offering conformational flexibility. They find applications in drug delivery, cancer treatment, chromatography, food industry, cosmetics, textiles, and industrial processes. They are also valuable in environmental science for removing pollutants and enhancing wastewater treatment. Concerns about CD toxicity have been addressed, primarily focusing on medical applications. CDs are versatile molecules with multifaceted applications across different fields, driven by their unique structural and chemical properties