Background: Diabetes mellitus is still one of the most important causes of death and disability worldwide. The therapeutic potential of saffron have been documented over the last two decades. The concept of that saffron has a possible anti-diabetic property is Fully-growing. Aim of the work: the aim of this study is to assess the possible therapeutic potential of aqueous saffron extract (ASE) in diabetic mice by investigating some physiological and biochemical parameters of different experimental groups. Material and Methods: A single dose of STZ (65 mg/kg body weight) were intraperitoneally injected to mice. After 24 hours, animals with fasting blood glucose over than 300 mg/dl were considered diabetic. The experimental groups were equally divided into; (1) Normal control, (2) Saffron, (3) Diabetic and (4) Diabetic + saffron. After the onset of diabetes, animals were treated with oral gavage of ASE (80 mg/kg body weight) along with drinking water. After 45 days from the end of the experimental period, measurement of blood glucose, body weight and lipid profile parameters were performed. Results: The current results recorded an obvious increase in blood glucose levels and parameters of lipid profile, together with a concomitant decrease in body weight in the diabetic group. However, treatment with ASE improved the profile of all the tested parameters towards the normal status as compared to untreated diabetic ones. Conclusion: The study suggests a therapeutic potential for ASE administration on the biochemical parameters in treated diabetic mice