The use of different types of synthetic pesticides has significantly contributed to the management of agricultural production losses caused by plant diseases. However, their use can induce numerous environmental and health problems which necessitate exploring safer solutions. Relying on plant biomass as a source of bioactive molecules can be an excellent alternative. In order to enhance the Hydro-methanolic extracts of the aerial parts (Leaves, Fruits) of Capparis spinosa L; As a potential source of bioactive molecules, having undergone two methods of extractions: Ultrasonic assisted extraction and maceration extraction. A phytochemical profile by FT-IR; HPLC-DAD; GC/MS; was established to better understand the different phytochemical components of the plant. The FT-IR analysis of the powders of the studied parts and their extracts revealed the presence of several chemical functions including the grouping of phenols. On this basis, this research focused on a quantification of phenolic compounds by HPLC-DAD, the majority being the catechol followed by coumaric respectively. The rate in the leaves extract from the maceration is (40.687 μg/g extract) while the rate in the fruits extract from the ultrasonic extraction is (41.326 μg/g extract). GC/MS analysis of the fractions obtained after silylation revealed the presence of 73 compounds for the Ethyl Acetate and Hexane fractions of the dry extracts of the studied leaves while noting the presence of 32 compounds in the Ethyl Acetate and Hexane fractions for the dried extracts of the fruits studied. The antimicrobial activity tested on reference strains showed a significant power of the dry extracts of the plant on the one hand against: Botrytis cinerea LPAP630. Fusarium oxysporum CTM 10402. Aspergillus niger CTM 10099 and on the other hand against: Agrobacterium tumefaciens B6. Agrobacterium tumefaciens C58. The results show the promising capabilities and efficiency of Capparis spinosa L in the field of biopesticide. Moreover, they highlight the open wide horizons for possible application through innovative bioprocesses for sustainable development.