This study investigates the synthesis, characterization, and antimicrobial properties of Lavender Extracted Zinc Nanoparticles (Lav ZnNPs) against cariogenic microorganisms. Certain bacteria are primary contributors to dental caries. Lavender's biofilm targeting capabilities, combined with the potent antimicrobial effects of zinc nanoparticles, offer a novel approach for caries prevention and treatment. Lav ZnNPs were synthesized and characterized using UV Vis spectroscopy and FTIR to confirm size, shape, and stability. Antibacterial efficacy was assessed through assays, showing significant inhibition of bacteria. Mol ecular docking studies were performed to understand the interactions between Lav ZnNPs and bacterial enzymes involved in biofilm formation. Results indicated strong binding affinities of Lav ZnNPs to glucosyltransferases and lactate dehydrogenase, critical for bacterial virulence and survival. These interactions suggest that Lav ZnNPs inhibit enzyme activity, impairing bacterial metabolism and biofilm development. This study highlights the synergistic effects of lavender and zinc nanoparticles, providing a foundation for developing advanced therapeutic strategies against dental caries through targeted experimental methods and bacterial inhibition.