Aim: Preliminary Phytochemical Investigation and comparative Quantitative Assessment of different extracts of Cuscuta reflexa, Murraya koenigii and Vitex negundo. Material and Methods: Plant material collected from the local region of Kangra, Himachal Pradesh. Extraction of plant material is done with the help of hot percolation with solvents of different polarity index. Percentage yield and phytochemical investigation of herbs were determined in different solvents. The total phenolic content and flavonoid content were determined by the Folin calcateaeu method and Aluminium chloride method respectively. Results: The percentage yield was more in hydroalcoholic solvent as compared to petroleum ether, chloroform and aqueous extracts of plant material. The phytochemical investigation reveals the presence of alkaloids, glycosides, phenols, flavonoids, tannins, carbohydrates in the extracts. The TPC and TFC of each extract when compared, it was found that hydroalcoholic extract of each plant have more content as compared to petroleum ether, chloroform and water extracts of the plants. Conclusion: The study concluded that the hydroalcoholic extracts of plants Cuscuta reflexa, Murraya koeinigii and Vitex negundo have majority of phytochemicals. The total phenolic and flavonoid content was more in hydroalcoholic extract of each plant as compared to petroleum ether, chloroform and water extract of the plants.