India is renowned as one of the world's top producers of livestock and milk. The cattle industry exerts significant influence on the economy, providing essential sustenance and diverse commodities to the populace. Livestock serve as vital sources of both nutrition and materials, extending beyond mere food production. However, a pressing concern within this sector is the prevalence of ticks, blood sucking parasites that pose a significant threat, drastically reducing the productivity of dairy animals and presenting formidable economic and welfare challenges in dairy farming. Historically, the prevailing approach involved the use of acaricides to eradicate attached larvae, nymphs, and adults. Nevertheless, the escalating proliferation of parasites, coupled with the emergence of resistant strains, has become increasingly prevalent. Hence, there is a crucial need to explore alternative systems that are cost-effective, environmentally friendly, and sustainable. Biological methods are preferred for their ability to ensure toxin-free milk and meat, thereby minimizing potential impacts on human health, the environment, and animal welfare. Essential oils offer a promising alternative due to their natural origin, therapeutic properties, and lower toxicity. Lemon grass oil (Cymbopogon citratus) has emerged as a particularly effective essential oil against ticks. This study primarily focuses on formulating lemon grass oil (Cymbopogon citratus) loaded banana starch nanoparticles to combat Rhipicephalus microplus in cattle. The particle size of starch and lemon grass oil-loaded starch nanoparticles was determined to be 431.6 and 527.1 nm, respectively, further confirmed using Scanning Electron Microscopy. The bio-efficacy of nano encapsulated lemon grass oil with starch nanoparticles was assessed using the Larval Packet Test (LPT) and Adult Immersion Test.