Background: Dengue fever is an emerging arboviral public health problem in a large endemic population in the tropical and sub-tropical areas of the world, with varying degrees of clinical presentation.All four dengue serotypes cause infection,with one of them predominantlyreported from a particular geographical region.This study was carried out to detect the predominant dengue serotype and presence of infections. Aim and Objectives: The present study was conducted to determine theSero-Prevalence of dengue serotyping cases among dengue patients infection in a tertiary care hospital, Pacific Institute of Medical Science, UdaipurRajasthan. Material and Methods: Acute-phase serum samples of patients suffering from dengue infection were collected.DengueNS1 antigen and IgM & IgG antibody against dengue virus detected by Rapid Dengue Duo Cassete kit and ELISA, and sample were carried out for serotyping of the dengue virus by the real time PCR. Results:A total of 210 samples were positive by the Enzyme linked immunoassay.85 were positive only for IgM, 45 were positive only for IgG and 80 samples were positive for both IgM and IgG antibodies. A total of 15 cases were found to have infection with more than one dengue serotypes. Only one serotypes DENV 2 were detected in these samples by PCR. DENV-2 was found to be most common circulating serotype. Samples having only antibodies and no antigen on rapid card test were also positive for virus by PCR. Conclusion: Prevalence of dengue infections is increasing. Moreover, it is important toscreen for dengue virus in those samples also which do not show NS1Ag on rapid tests andhave either one or both the antibodies. Real-time PCR is found to be more sensitive in detecting infection than Enzyme linked immunoassay.