Methicillin resistant Staphylococcus aureus (MRSA) is constantly occur and is a major trouble for tertiary care centre. Aims of this study are detection prevalence rate and antibiotics sensitivity of MRSA in our tertiary care centre. Material & method: Present study was undertaken from January 2021 to December 2022, at the Clinical Microbiology Laboratory, Dhiraj Hospital, Piparia. The entire clinical specimens were processed as per laboratory standard protocol. Indentified of organisms and antimicrobial susceptibility was done by biochemical reaction and automated VITEK 2 compact system. Out of all isolated organisms only Staphylococcus aureus (S. aureus) were included in this study. Screening for MRSA was done by cefoxitin disc diffution test, cefoxitin and oxacillin screening done also by automated VITEK 2 compact system (BioMérieux, France). Result: During study period, S. aureus were isolated from 257 different samples; which include pus and wound swab, blood, sputum, urine and Miscellaneous ( Body fluid, endotracheal secretion, tissue, implants), out of this 117 (45.53%) were identified as MRSA. Most of MRSA cases in old age patients (>60 years of age) which was 36.8%. Hospital admitted cases affected mostly with MRSA which was 71.8% with highest in isolates from pus samples (57.3%) and from orthopedics ward (39.3%). Vancomycin, linezolid, and teicoplanin were highest sensitivity against MRSA which were 100%, 93.2% and 94.9% respectively. Conclusion: Screening of MRSA and their antibiogram in tertiary care hospital is very essential for early detection of MRSA and for management of the condition.