Dentistry stands out as one of the most challenging professions, as evidenced by research indicating that stress levels increase as academic complexity intensifies with advancing courses. The aim is to determine the prevalence of stress among students at the Faculty of Dentistry of the Catholic University of Cuenca, Azogues campus. The research was observational, descriptive, and cross-sectional, in which the Dental Environment Stress Questionnaire (DES30) was applied. The study population consisted of 403 students, and the final sample comprised 306 learners. The results highlight high levels of stress among dental students, such as fear of failing a course or a year (57.8%), lack of time for relaxation (38.6%), assessments and grades (38.9%), financial responsibilities (36.6%), patients arriving late or missing appointments (35.6%), and meeting graduation requirements (30.1%). It is concluded that the prevalence of stress varies among the various sources evaluated. Some sources, such as "fear of failing a course or a year," "assessments and grading," "number of homework assignments in class," "financial responsibilities," and "lack of time to complete assigned tasks," show notably high prevalence rates, between 89% and 96%.