Production and optimization of culture medium composition as well as purification and characterization of alpha amylase from Aspergillus niger using TME 419 cassava peels as substrate was carried out during the course of this study. Proximate analysis of the dry weight cassava peels was 10.08% moisture content, 6.60% ash content, 11.02% crude fiber, 4.91% crude protein, 1.58% ether extract (fat) and 75.83% total carbohydrate