Ethanol produced by the use of fermentation of different biological materials is a clear liquid alcohol. As a fuel, bioethanol has significant characteristics, including a high octane number and rapid vaporization rate. In the present day, bioethanol is the most commonly produced biofuel, mainly derived from the first generation, resulting in a competition between food and biofuel. Many developing countries are currently facing this challenge while lignocellulosic waste is not being utilized. Since the second generation of bioethanol production uses non-edible feedstock derived from agricultural, municipal, domestic and forestry waste, it fills the gap created by the first generation of bioethanol production. The major aim of this study was to compare the yield of ethanol produced by Saccharomyces cerevisiae from three different types of lignocellulosic biomasses: sugarcane bagasse, paper waste and domestic waste. Sugarcane bagasse, domestic wastes were dried and ground to powder and pretreated with 4% H2SO4 at 60℃ for 2 hours followed by the hydrolysis using cellulase enzyme. The paper waste was cut into pieces, hydrolyzed using 5% H2SO4 at 121℃ for 120 mins. The composition of the substrates was analyzed before and after pretreatment. All the three substrates were fermented for 72 hours at 35℃. Among all these tested substrates, paper waste produced maximum ethanol followed by sugarcane bagasse and domestic waste.