Cassia fistula belongs to the family Caesalpinaceae commonly known as “Golden shower tree” has been used in different traditional system of medicines for various ailments since ancient times. Cassia fistula grows throughout in Bangladesh and in many other Asian countries such as India, China, Hong Kong, Philippines, Malaysia, Indonesia, and Thailand. Quantitative estimation of phytoconstituents present in ethanolic (EE), Aqueous (AE) and Hydroalcholic (HAE) leaf extract of Cassia fistula. Ethanolic leaf extract of Cassia fistula contain 54.53 mg total tannin content, 8.31 mg total phenolic content, 204.6 mg of total Alkaloidal content, 296.8 mg total flavanoids content. Aqueous leaf extract of Cassia fistula contain 10.61 mg total tannin content, 4.50 mg total phenolic content, 98.0 mg of total Alkaloidal content, 85.5 mg total flavonoid content. Hydroalcholic leaf extract of Cassia fistula contain 25.03 mg total tannin content, 6.10 mg total phenolic content, 119.11 mg of total Alkaloidal content, 118.25 mg total flavanoids content. This study showed that the ethanolic leaf extract of Cassia fistula contain high percentage of tannins, Phenols, Flavanoids and alkaloids.