Antibiotics used in breeding are susceptible of being found in meat and poultry products thus causing a risk to public health. The aim of this study is to investigate antimicrobial residues in poultry livers. A total of 82 samples (including 50 chicken livers and 32 turkey livers) were collected randomly from different butchers in Souk Ahras province and analyzed by the microbiological method of agar diffusion using three susceptible bacterial strains namely: Staphylococcus aureus ATCC 23, Pseudomonas aeruginosa ATCC 53 and Escherichia coli ATCC 22. The study revealed that out of 82 samples of livers analyzed, 54 livers were contaminated with antibiotic residues, with an average contamination rate of 65.85%. For broiler chicken, the results showed that 90% containing Quinolones (enrofloxacin) ,58% of Tetracyclines ,88% of Macrolides (erythromycin) and 33% of Polypeptide antibiotics (colistin). For turkey livers, the results showed that 28.13% contained Macrolides (erythromycin) , 25% Quinolones (enrofloxacin), 12.5% Polypeptide antibiotic (colistin) and 6 .25% Tetracycline. The results show that the contamination rate is higher for chiken livers than for turkey ones.