Prostate cancer remains a challenge in healthcare, being second most common male cancer demanding innovative therapeutic approaches and treatment techniques. This study investigatedin vitromethodsfor the investigation of the potential anticancer effects of quercetin loaded and alginate-sealed β-Glucan particles derived from yeast against the DU145 cell line. In vitro evaluation using MTT assays demonstrated the cytotoxic effects of quercetin loaded and alginate-sealed particles against DU145 prostate cancer cells. Apoptosis induction, ROS generation, and lysosomal pH alterations underscore the potential of quercetin loaded and alginate sealed β-Glucan particles as promising therapeutic agent for prostate cancer. Future studies focusing on preclinical validation, pharmacokinetic profiling, and clinical trials to assess translational potential and optimize therapeutic strategies can help to use Quercetin loaded β-Glucan as an therapeutic option for the treatment of prostate cancer.