The hoverflies Eristalinus quinquestriatus and Melanostoma orientale both were reported from the Uttar Pradesh state but not from the specific area or city. Eristalinus quinquestriatus is the species belongs to subfamily Eristalinae is treated as forgotten species because its record from Uttar Pradesh is not constant according to the provided literature. Whereas Melanostoma orientale is constantly reported from the state. Here is an attempt to mark their presence from Gorakhpur city of Uttar Pradesh by DNA sequencing as it is the most reliable and efficient method of proper identification of species till the date. Detailed taxonomic characters, DNA report, distribution and photographs are given in this article. The analysed sequence is submitted to the National Centre of Biotechnology and Information (NCBI).