Periodontal diseases consist of various conditions that affect the periodontal tissues and their integrity. Diagnosis of periodontal conditions and defects that occur as sequelae of periodontitis, like infra-bony defects, dehiscence, and fenestration, requires thorough clinical and radiographic examinations. With the introduction of 3D imaging, image manipulation and navigation using specialized software were made possible for obtaining high-quality images, leading to better diagnosis. The introduction of CBCT has brought a series of advantages to the field of diagnostic dental imaging over conventional radiographic techniques, including the elimination of distortion and the ability to visualize structures in all planes, in addition to its lower radiation dose, user-friendly software, accuracy, and availability in comparison to CT. Aim: In the present study, the aim was to compare two different resolutions of CBCT in terms of diagnostic performance, accuracy, and reliability in specific circumstances like small periodontal defects and lesions. Materials and methods: a total of 79 periodontal defects, resembling infra-bony defects (IBD), dehiscence, and fenestrations, were created using round burs of different sizes on eight dry human skulls. The linear measurements of each defect were recorded using a digital caliper and were considered the ‘gold standard’.