Organic matter is essential for improving soil fertility and ensuring long-term soil sustainability. In contrast to chemical fertilizers, they have the potential to improve the overall condition of the soil, offering benefits both now and in the future. The current experiment investigated the release of four enzymes: Dehydrogenase, β-Glucosidase, Urease, and Alkaline phosphatase, in an intermediate manner at different stages of crop growth. The release pattern of enzymes was examined for 11 organic treatments over 30, 60, 90 and 120 days after crop sowing. A two-way analysis of variance (ANOVA) and Duncan’s Multiple Range Test (DMRT) were conducted for all four enzymes, using a 95% significance level (p<0.05) to evaluate the significant differences among the various treatments. Treatment T9 (75% Vermicompost + 75% PressMud) demonstrated the most favorable outcomes for all four enzymes, followed by treatment T11 (75% PressMud + 75% Farmyard manure) and T7 (75% Vermicompost + 75% Farmyard manure). The percent change was also significant in the organically amended plots.