Triclosanis a broad-spectrum antibiotic that is today used in many consumer and medical items, such as vicryl suture, surgical scrubs, and toothpaste (Bhargava and Leonard, 1996). Waters C18 (4.6 x 250 mm, 5 µm) Column Detector Wavelength: 280 nm in UV, Eluent A: acetonitrile Eluent: B 0.1% orthophosphoric acid in water as mobile phase in the ratio of (80:20). The flow rate is 0.8 ml/min with ambient temperature of column. Sample preparation with Methanol: Water of HPLC quality 80:20 ratio. The method used was verified in compliance with ICH regulations. With a correlation value of 0.999, the results showed good linearity. The retention time (Rt) was determined to be 4.6 ± 0.4 It was discovered that the technique was straightforward, specific TCS in dental formulations and for regular quality control assessments.