The increasing population along with urbanization and industrialization hasaugmentedwith a drastic accumulation of hazardous heavy metal wastes into the milieuposing potentialhazards to human health andthe environment. In comparison to chemical and physical remediation, microbial bioaccumulation is cost-effective and eco-friendly.Current investigation involved the screening and characterization of heavy metal-tolerantbacterial isolates fromindustrial-polluted soil samples around Bangalore.Of the 184bacterial colonies screened, 28isolates were characterized due to their significant growth in Luria Bertini (LB)containing100ppm of lead (Pb), cadmium (Cd), and chromium (Cr).The analysis exhibited isolates from all three soil sampleswithhighest resistance towards heavy metals as Pb, followed by Cr and Cd i.e., Pb>Cr>Cd. Majority were observed to bespore forming Gram-positive bacillifollowed by Gram-negative bacteria.Subsequently, MALDI-TOF-MS identificationrevealedgenus Bacillus as predominant species, followed by Staphylococcus sp, Pseudomonas, Acinetobacter, Enterococcus, Citrobacter,andPaenibacillus.Heavy metal tolerance revealed, 3000ppm for Pb as highest, followed by 2200ppm for Cr and 700 for Cd. The highest metal tolerance was observed inB. megateriumwith MIC values of 3000ppm, 1000ppm, and 1500ppm for Pb, Cd and Cr respectivelyindicating presence of multiple genes for remediation. However, significant results were revealed for Cr metal tolerance at 2200ppm by S. xylosus,B. subtilisand B. ceruesshowed Cd tolerance of 2200ppm. Among Gram-negative bacteria, Pseudomonas aeruginosa and Citrobacter youngae showed substantial Pb and Cr resistance at2500ppm and1000ppmrespectively and without much significance towards Cd. Current study highlights the potential soil bacteria withsignificant heavy metaltolerance that could be efficiently employed to develop sustainable bioremediationwithcompetentto restore ecological balance in heavy metal affected zones.