In the current research investigation on the plant of Cucurbitaceae family , the Anti-microbial activity and Anthelmintic activity of the plant Cucumis callosus was investigated. The Ethanolic extract taken of the fruit pulp of the plant showed antibacterial activity only against Staphylococcus aureus and Klebsiella pneumonia. Data also revealed that decrease in concentration of extract either lowers activity or eliminate the activity as shown mostly in case of concentration 1.25 mg in most of the cases. In the Anthelmintic activity investigation of of plant Cucumis callosus it was found from the results that the aqueous extract exhibited more potent anthelmintic activity (paralysis time 2-3 min and death time 3-5 min) even more than standard drug while the ethanolic extract has taken long time for death of worms