A powerful uricase producing actinobacteria was discovered as Streptomyces ennisocaselis var A85 after a comprehensive screening process. Utilizing response surface methodological optimization, the newly discovered Streptomyces strain produced more uricase. Plackett Burman (PB) design was utilized in media optimization studies to select the essential media components, which were then further optimized through the application of central composite design (CCD). Sucrose, uric acid and NaCl were discovered to be the key elements affecting the production of enzyme uricase. Through the use of central composite design, the best uricase production with these parameters was determined. Significant factors were of sucrose- 1.25g, peptone - 0.25g, NaCl - 0.03g, Na2HPO4 - 0.05g, CaCl2 - 0.025g, uric acid - 0.107g, MgSO4.7H2O - 0.025 for 50 mL of production medium. The utilization of statistical optimization resulted in a significant increase in uricase production, raising it from 3.33 to 17.00 U/mL. Overall, there was a 5.15-fold increase in uricase production accomplished by using the optimized medium with strain A85.