An investigation was executed to induce mutations and work out the effective dose of physical and chemical mutagen under varied concentration and isolate desirable mutants in two cultivars viz American beauty and Nova Lux were screened in two consecutive years in two generations M1 and M2. Study was laid out in factorial randomized block design during Rabi season in 2020 and 2021 in the Department of Horticulture, Janta College, Bakewar, Etawah in three replications.The standard sized corms of two cultivars of gladiolus were treated with physical mutagen of dosage of 60Cogamma irradiation source (1kR, 2 kR, 3 kR, 4 kR and 5 kR)and Ethyl methane sulfonate (EMS) (0.4%, 0.6% and 0.8%). Scrutiny of the data on floral characters in each treatment was done to assess the influence of varied mutagenic treatments. The results depicted that Significantly minimum (56.13 and 64.46) days were required for the initiation of spike, more number of 2.20 and 1.86 spikes, length of spike with 71.68 cm and 76.65 cm, higher number of 13.82 and 14.48 florets per spike, maximum diameter of 10.23 cm and 10.74 cm, floret length of 9.41 cm and 10.58 cm, and higher vase life of 9.14 and 10.87 days of cut spike in M1 generation in American beauty and Nova lux cultivar of gladiolus under the treatment comprising of corms treated with gamma dosage @ 2kR (T3), respectively. Minimum number of spikes, length of spike, diameter of floret, and vase life of cut spikes were expressed from treatment T6 (gamma irradiation @ 5kR) in vM1 for American beauty cultivar and Nova Lux. Therefore, it can be concluded that floral attributes were better with the application of lower dosage of gamma radiations.